Anti-ICAM-1 Antibody

Cat: 10346-MM03
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Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-ICAM-1 Antibody
Validated applications
WB
Species reactivity
Reacts with: Human
Specificity
Human ICAM-1
No cross-reactivity in ELISA with Human ICAM2 / CD102 Human ICAM3 / CD50,Human VCAM1 / CD106 Human CEACAM1 / CD66a Human E-selectin / SELE / CD62E / ELAM1
Immunogen
Recombinant Human ICAM-1 protein (Catalog#10346-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 extracellular domain (rh ICAM1; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #8H8G9
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ICAM-1 Antibody (Mouse Monoclonal antibody) Images
Human ICAM-1/CD54 Western blot (WB) 13195

Anti-ICAM1 mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:65 kDa

Anti-ICAM-1 Antibody Alternative Names
Anti-BB2 Antibody;Anti-CD54 Antibody;Anti-ICAM-1 Antibody;Anti-P3.58 Antibody
ICAM-1 Background Information

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Full Name
intercellular adhesion molecule 1
Research Areas
References
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA(Det) 50440-R101
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    Anti-ICAM-1 Antibody Rabbit Monoclonal IHC-P 50440-R280
    Anti-ICAM-1 Antibody Rabbit Monoclonal FCM 50440-R009
    Anti-ICAM-1 Antibody (PE) Rabbit Monoclonal FCM 50440-R009-P
    Anti-ICAM-1 Antibody (FITC) Rabbit Monoclonal FCM 50440-R009-F
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA 10346-R013
    Anti-ICAM-1 Antibody (PE) Mouse Monoclonal FCM 10346-MM01-P
    Anti-ICAM-1 Antibody (PerCP) Mouse Monoclonal FCM 10346-MM01-C
    Anti-ICAM-1 Antibody (FITC) Mouse Monoclonal FCM 10346-MM01-F
    Anti-ICAM-1 Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM,ICC/IF,IF 10346-MM01
    Anti-ICAM-1 Antibody Mouse Monoclonal ELISA 10346-MM04
    Anti-ICAM-1 Antibody (APC) Mouse Monoclonal FCM 10346-MM01-A
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10346-T26
    Anti-ICAM-1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10346-RP02
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 10346-T16
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 10346-RP01
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