|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
ICAM1 was immunoprecipitated using:
Lane A:0.5 mg Raji Whole Cell Lysate0.5 µL anti-ICAM1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-ICAM1 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 95 kDa
Observed band size: 95 kDa
Anti-ICAM1 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:58 kDa
Observed band size:58 kDa
(We are unsure as to the identity of these extra bands.)
Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-ICAM-1 Antibody (FITC)||Rabbit||Monoclonal||FCM||80022-R037-F|
|Anti-ICAM-1 Antibody (PE)||Rabbit||Monoclonal||FCM||80022-R037-P|
|Anti-ICAM-1 Antibody (APC)||Rabbit||Monoclonal||FCM||80022-R037-A|
|Anti-ICAM-1 Antibody (PE)||Rabbit||Monoclonal||FCM||50440-R009-P|
|Anti-ICAM-1 Antibody (FITC)||Rabbit||Monoclonal||FCM||50440-R009-F|
|Anti-ICAM-1 Antibody (PE)||Mouse||Monoclonal||FCM||10346-MM01-P|
|Anti-ICAM-1 Antibody (PerCP)||Mouse||Monoclonal||FCM||10346-MM01-C|
|Anti-ICAM-1 Antibody (FITC)||Mouse||Monoclonal||FCM||10346-MM01-F|
|Anti-ICAM-1 Antibody (APC)||Mouse||Monoclonal||FCM||10346-MM01-A|