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ICAM-1/CD54 FITC Rabbit MAb

  • ICAM-1 / CD54 Antibody (FITC), Rabbit MAb, Flow cytometric
  • Rat ICAM-1/CD54 Flow Cytometry (FC) 4829
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Rat ICAM1 Antibody Product Information
Immunogen:Recombinant Rat ICAM-1 / CD54 protein (Catalog#80022-R08H)
Clone ID:037
Ig Type:Rabbit IgG
Concentration:2 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; Catalog#80022-R08H; Q00238-1; Met1-Thr493) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Rat ICAM1 Antibody FC Application Image
ICAM-1 / CD54 Antibody (FITC), Rabbit MAb, Flow cytometric
[Click to enlarge image]
Caption:
Analysis of Rat ICAM1(CD54) expression on spleen lymphocytes. Crossreactivity on both rat and mouse splenocytes is observed. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
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                Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

                Lane A: NIH3T3 Whole Cell Lysate

                Lane B: Hela Whole Cell Lysate

                Lysates/proteins at 30 μg per lane.

                Secondary

                Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

                Developed using the ECL technique.

                Performed under reducing conditions.

                Predicted band size:15 kDa

                Observed band size:17 kDa

                ICAM-1/CD54 Background

                Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

                Rat ICAM-1/CD54 References
              • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
              • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
              • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
              • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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                Catalog: 80022-R037-F-100
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                100 Tests
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                Datasheet & Documentation

                All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"