Human IAPP Gene cDNA Clone / ORF Clone

IAPP cDNA Clone Datasheet

IAPP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000415.2
• cDNA Size: 270
• Gene Synonym: DAP; IAP; IAPP
• Source: Human

IAPP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens islet amyloid polypeptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 131 G/C, 250-251 CT/GG resulting in the amino acid Arg substitution by Pro, Leu substitution by Gly and 69 A/C not causing the amino acid variation.

IAPP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IAPP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IAPP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCATCC TGAAGCTGCA AGTATTTCTC ATTGTGCTCT CTGTTGCATT GAACCATCTG
61       AAAGCTACCC CCATTGAAAG TCATCAGGTG GAAAAGCGGA AATGCAACAC TGCCACATGT
121      GCAACGCAGC CCCTGGCAAA TTTTTTAGTT CATTCCAGCA ACAACTTTGG TGCCATTCTC
181      TCATCTACCA ACGTGGGATC CAATACATAT GGCAAGAGGA ATGCAGTAGA GGTTTTAAAG
241      AGAGAGCCAG GGAATTACTT GCCCCTTTAG

 

Quality Control:cDNA sequencing results is available upon request.