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Human IAPP Gene cDNA Clone (full-length ORF Clone)
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Human IAPP Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13736-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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IAPP cDNA Clone Datasheet

 

IAPP cDNA Clone Product Information

Gene Bank Ref.ID: NM_000415.2

cDNA Size:

270

Gene Synonym:

DAP; IAP; IAPP

Source: Human

IAPP cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens islet amyloid polypeptide DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 131 G/C, 250-251 CT/GG resulting in the amino acid Arg substitution by Pro, Leu substitution by Gly and 69 A/C not causing the amino acid variation.

IAPP cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IAPP cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IAPP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCATCC TGAAGCTGCA AGTATTTCTC ATTGTGCTCT CTGTTGCATT GAACCATCTG
61       AAAGCTACCC CCATTGAAAG TCATCAGGTG GAAAAGCGGA AATGCAACAC TGCCACATGT
121      GCAACGCAGC CCCTGGCAAA TTTTTTAGTT CATTCCAGCA ACAACTTTGG TGCCATTCTC
181      TCATCTACCA ACGTGGGATC CAATACATAT GGCAAGAGGA ATGCAGTAGA GGTTTTAAAG
241      AGAGAGCCAG GGAATTACTT GCCCCTTTAG

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"