Human coronavirus spike glycoprotein Antibody

Human coronavirus spike glycoprotein Antibody Datasheet

Human coronavirus spike glycoprotein Antibody Product Information

• Immunogen: Recombinant Human coronavirus spike glycoprotein protein (Catalog#40021-V08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 07
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human coronavirus spike glycoprotein (Catalog#40021-V08H; YP_173238.1; Met 1-Arg 760). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Human coronavirus spike glycoprotein Antibody Usage Guide

• Specificity: Human coronavirus spike glycoprotein
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human coronavirus spike glycoprotein. The detection limit for Human coronavirus spike glycoprotein is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Human coronavirus spike glycoprotein Antibody Background

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. In 2003, a novel coronavirus which is known as SARS coronavirus cause the severe acute respiratory syndrome (SARS) epidemic that began in Asia in 2002. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits: the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.

References

1. Shen S, et al. (2007) Methods Mol Biol. 379: 127-35.
2. Du L, et al. (2009) 7 (3): 226-36.
3. Xiao X, et al. (2004) Cell Mol Life Sci. 61 (19-20): 2428-30.