WT1 qPCR Primer Pairs, Human

Cat: HP101890
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WT1 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
WT1 qPCR Primer Pairs, Human Alternative Names
AWT1 qPCR Primer Pairs, Human;EWS-WT1 qPCR Primer Pairs, Human;GUD qPCR Primer Pairs, Human;NPHS4 qPCR Primer Pairs, Human;WAGR qPCR Primer Pairs, Human;WIT-2 qPCR Primer Pairs, Human;WT33 qPCR Primer Pairs, Human
WT1 Background Information

WT1 gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG.

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Full Name
Wilms tumor 1
References
  • Niavarani A, Herold T, Reyal Y, et al. A 4‐gene expression score associated with high levels of Wilms Tumor‐1 (WT1) expression is an adverse prognostic factor in acute myeloid leukaemia. British Journal of Haematology. 2016;172(3):401-411.
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