VSIG4 qPCR Primer Pairs, Human

Cat: HP101797
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VSIG4 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
PCR Size:
184bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
VSIG4 qPCR Primer Pairs, Human Alternative Names
CRIg qPCR Primer Pairs, Human;Z39IG qPCR Primer Pairs, Human
VSIG4 Background Information

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I  transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 280 amino acid precursor that contains a signal sequence, an V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.

Full Name
V-set and immunoglobulin domain containing 4
References
  • Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826.
  • Helmy, K. et al., 2006, Cell. 124:915-927.
  • Wiesmann, C. et al., 2006, Nature. 444:217-220.
  • Zang,X. et al., 2006, J Clin Invest. 116: 2590-2593.
  • Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325.
  • He,JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
  • VSIG4 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    VSIG4 cDNA ORF Clone in Cloning Vector, Mouse MG50187-M
    VSIG4 cDNA ORF Clone, Mouse, untagged MG50187-UT
    VSIG4 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50187-ACGLN
    VSIG4 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50187-ACG
    VSIG4 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50187-ACR
    VSIG4 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50187-CF
    VSIG4 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50187-NF
    VSIG4 cDNA ORF Clone, Mouse, C-His tag MG50187-CH
    VSIG4 cDNA ORF Clone, Mouse, N-His tag MG50187-NH
    VSIG4 cDNA ORF Clone, Mouse, C-HA tag MG50187-CY
    VSIG4 cDNA ORF Clone, Mouse, N-HA tag MG50187-NY
    VSIG4 cDNA ORF Clone, Mouse, C-Myc tag MG50187-CM
    VSIG4 cDNA ORF Clone, Mouse, N-Myc tag MG50187-NM

    VSIG4 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    VSIG4 cDNA ORF Clone in Cloning Vector, Human HG12163-G
    VSIG4 cDNA ORF Clone, Human, untagged HG12163-UT
    VSIG4 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG12163-ACGLN
    VSIG4 cDNA ORF Clone, Human, C-GFPSpark® tag HG12163-ACG
    VSIG4 cDNA ORF Clone, Human, C-OFPSpark® tag HG12163-ACR
    VSIG4 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12163-G-F
    VSIG4 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12163-CF
    VSIG4 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12163-NF
    VSIG4 cDNA ORF Clone, Human, C-His tag HG12163-CH
    VSIG4 cDNA ORF Clone, Human, N-His tag HG12163-NH
    VSIG4 cDNA ORF Clone, Human, C-HA tag HG12163-CY
    VSIG4 cDNA ORF Clone, Human, N-HA tag HG12163-NY
    VSIG4 cDNA ORF Clone, Human, C-Myc tag HG12163-CM
    VSIG4 cDNA ORF Clone, Human, N-Myc tag HG12163-NM
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