|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VDR ORF mammalian expression plasmid, C-GFPSpark tag||HG12025-ACG|
|Human VDR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12025-ACR|
|Human VDR ORF mammalian expression plasmid, N-GFPSpark tag||HG12025-ANG|
|Human VDR ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12025-ANR|
|Human VDR ORF mammalian expression plasmid, C-Flag tag||HG12025-CF|
|Human VDR ORF mammalian expression plasmid, C-His tag||HG12025-CH|
|Human VDR ORF mammalian expression plasmid, C-Myc tag||HG12025-CM|
|Human VDR ORF mammalian expression plasmid, C-HA tag||HG12025-CY|
|Human VDR Gene cDNA clone plasmid||HG12025-G|
|Human VDR natural ORF mammalian expression plasmid||HG12025-G-N|
|Human VDR ORF mammalian expression plasmid, N-Flag tag||HG12025-NF|
|Human VDR ORF mammalian expression plasmid, N-His tag||HG12025-NH|
|Human VDR ORF mammalian expression plasmid, N-Myc tag||HG12025-NM|
|Human VDR ORF mammalian expression plasmid, N-HA tag||HG12025-NY|
|Human VDR natural ORF mammalian expression plasmid||HG12025-UT|
|Learn more about expression Vectors|
VDR (vitamin D(1,25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1,25(OH)2D3) and induce expression of the 1,25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.