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Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tag

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    Human USH1C cDNA Clone Product Information
    NCBI RefSeq:NM_005709.2
    RefSeq ORF Size:1659bp
    cDNA Description:Full length Clone DNA of Homo sapiens Usher syndrome 1C (autosomal recessive, severe), transcript variant 1 with N terminal Flag tag.
    Gene Synonym:PDZ73, AIE-75, DFNB18, PDZ-45, PDZ-73, NY-CO-37, NY-CO-38, ush1cpst, PDZ-73/NY-CO-38
    Species:Human
    Vector:pCMV3-N-FLAG
    Plasmid:
    Restriction Site:
    Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Sequence Description:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with USH1C qPCR primers for gene expression analysis, HP100863 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tag on other vectors
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10613-ACG$245
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10613-ACR$245
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10613-ANG$245
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10613-ANR$245
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10613-CF$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tagHG10613-CH$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10613-CM$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA tagHG10613-CY$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10613-M$75
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10613-M-F$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10613-NF$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His tagHG10613-NH$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10613-NM$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tagHG10613-NY$215
    Human USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmidHG10613-UT$215
     Learn more about expression Vectors
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    Background

    Harmonin, also known as Antigen NY-CO-38 / NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein and USH1C, is a protein which is expressed in small intestine, colon, kidney, eye and weakly in pancreas. USH1C is expressed also in vestibule of the inner ear. USH1C contains 3 PDZ (DHR) domains. USH1C may be involved in protein-protein interaction. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C), also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). Defects in USH1C are also the cause of deafness autosomal recessive type 18 (DFNB18) which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

    References
  • Verpy, E. et al., 2000, Nat Genet. 26 (1):51-5.
  • Weil D., et al., 2003, Hum. Mol. Genet. 12:463-471.
  • Reiners,J. et al., 2005, Hum Mol Genet. 14 (24):3933-43.
  • Yan,D. et al., 2006, Mol Biol. 357 (3):755-64.
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    Catalog: HG10613-NF
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"