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Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tag

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Human UNG cDNA Clone Product Information
NCBI RefSeq:NM_003362.2
RefSeq ORF Size:915bp
cDNA Description:Full length Clone DNA of Homo sapiens uracil-DNA.glycosylase, transcript variant 1 with C terminal His tag.
Gene Synonym:DGU, UDG, UNG1, UNG2, HIGM4, UNG15, DKFZp781L1143, UNG
Species:Human
Vector:pCMV3-C-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tag on other vectors
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG12117-ACG$225
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG12117-ACR$225
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG12117-ANG$225
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG12117-ANR$225
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG12117-CF$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tagHG12117-CH$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tagHG12117-CM$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA tagHG12117-CY$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone in cloning vectorHG12117-M$75
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tagHG12117-NF$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His tagHG12117-NH$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc tagHG12117-NM$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tagHG12117-NY$195
Human uracil-DNA glycosylase transcript variant 1 Gene ORF cDNA clone expression plasmidHG12117-UT$195
 Learn more about expression Vectors
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Background

Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. Uracil-DNA glycosylase exists in two forms: mitochondrial uracil-DNA glycosylase 1 (UNG1) and nuclear uracil-DNA glycosylase 2 (UNG2). uracil-DNA glycosylase. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.

References
  • Akbari M, et al. (2007) Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stress. Neuroscience. 145(4):1201-12.
  • Slupphaug G, et al. (1996) Properties of a recombinant human uracil-DNA glycosylase from the UNG gene and evidence that UNG encodes the major uracil-DNA glycosylase. Biochemistry. 34(1): 128-38.
  • Pytel D, et al. (2008) Uracil-DNA glycosylases. Postepy Biochem. 54(4): 362-70.
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