TPSB2 cDNA ORF Clone, Human, N-HA tag General Information
Full length Clone DNA of Human tryptase beta 2 with N terminal HA tag.
Enhanced CMV promoter
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
TPSB2 cDNA ORF Clone, Human, N-HA tag Alternative Names
TPS2 cDNA ORF Clone, Human;tryptaseB cDNA ORF Clone, Human;tryptaseC cDNA ORF Clone, Human
TPSB2 Background Information
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1, and fall into two groups, α and β. β-tryptases appear to be the main isoenzymes expressed in mast cells, whereas α-tryptases predominate in basophils. Tryptase is unique in two respects: it is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known endogenous proteinase inhibitors because of the unique arrangement of the active sites. Additionally, tryptase family genes have an intron immediately upstream of the initiator codon which separates the transcription initiation site from protein coding sequence, and this feature is characteristic of tryptases. β-tryptases existing in three isoforms (β1,β2,β3) are released in secretory granules, and have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. It has been reported that β-tryptase selectively cleaves ASM-derived eotaxin and RANTES and abrogates their chemotactic activities.
tryptase beta 2 (gene/pseudogene)
Miller, J.S. et al., 1990, J. Clin. Invest. 86: 864-870. Pereira, P.J. et al., 1998, Nature. 392: 306-311. Pallaoro, M.et al., 1999, J. Biol. Chem. 274: 3355-3362. Pang, L. et al., 2006, J. Immunol. 176: 3788-3795.