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Human TNFR2 / CD120b / TNFRSF1B Human Cells Transfected Lysate (positive control) (denatured)
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Human TNFR2 / CD120b / TNFRSF1B Human Cells Transfected Lysate (positive control) (denatured) PDF Download

Catalog   Size (Price) Quantity In Stock Operation
10417-H03HL  
YES   
 

    Notice: Additional handling time (approximately 2 weeks) may be required after placing the order.

Human TNFRSF1B / TNFR2 / CD120b Human Cells Transfected Lysate Datasheet

 

Human TNFRSF1B / TNFR2 / CD120b Human Cells Transfected Lysate Product Information

Products Description: Human Cells transfected lysate in which Human TNFRSF1B / TNFR2 / CD120b has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Browse all transfected cell lysate positive controls
Host: Human

Human TNFRSF1B / TNFR2 / CD120b Human Cells Transfected Lysate QC Testing

Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue

Human TNFRSF1B / TNFR2 / CD120b Human Cells Transfected Lysate Usage Guide

Recommend Usage:

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.

2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.

3. Store it at -80℃. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.

Storage Buffer: In modified RIPA Lysis Buffer
Storage Instruction: Store at -80℃. Aliquot to avoid repeated freezing and thawing
Stability: Samples are stable for up to twelve months from date of receipt at -80℃

Human TNFRSF1B / TNFR2 / CD120b Human Cells Transfected Lysate Applications

Application notes:

WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"