|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TNFRSF17 ORF mammalian expression plasmid, C-GFPSpark tag||HG10620-ACG|
|Human TNFRSF17 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10620-ACR|
|Human TNFRSF17 ORF mammalian expression plasmid, N-GFPSpark tag||HG10620-ANG|
|Human TNFRSF17 ORF mammalian expression plasmid, C-Flag tag||HG10620-CF|
|Human TNFRSF17 ORF mammalian expression plasmid, C-His tag||HG10620-CH|
|Human TNFRSF17 ORF mammalian expression plasmid, C-Myc tag||HG10620-CM|
|Human TNFRSF17 ORF mammalian expression plasmid, C-HA tag||HG10620-CY|
|Human TNFRSF17 ORF mammalian expression plasmid, N-Flag tag||HG10620-NF|
|Human TNFRSF17 ORF mammalian expression plasmid, N-His tag||HG10620-NH|
|Human TNFRSF17 ORF mammalian expression plasmid, N-Myc tag||HG10620-NM|
|Human TNFRSF17 ORF mammalian expression plasmid, N-HA tag||HG10620-NY|
|Human TNFRSF17 natural ORF mammalian expression plasmid||HG10620-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.