Tim4 qPCR Primer Pairs, Human

Cat: HP101795
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Tim4 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Tim4 qPCR Primer Pairs, Human Alternative Names
SMUCKLER qPCR Primer Pairs, Human;TIM4 qPCR Primer Pairs, Human
Tim4 Background Information

A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule; also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factor-alpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.

Full Name
T-cell immunoglobulin and mucin domain containing 4
References
  • Miyanishi M, et al. (2007) Identification of Tim4 as a phosphatidylserine receptor. Nature. 450(7168): 435-9.
  • Feng BS, et al. (2008) Disruption of T-cell immunoglobulin and mucin domain molecule (TIM)-1/TIM4 interaction as a therapeutic strategy in a dendritic cell-induced peanut allergy model. J Allergy Clin Immunol. 122(1): 55-61.
  • Cai PC, et al. (2009) Association of TIM4 promoter polymorphism -1419GA with childhood asthma in a Chinese Han population. Tissue Antigens. 74(1): 11-6.
  • Zhao CQ, et al. (2010) Specific immunotherapy suppresses Th2 responses via modulating TIM1/TIM4 interaction on dendritic cells. Allergy. 65(8): 986-95.
  • Tim4 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    Tim4 cDNA ORF Clone in Cloning Vector, Mouse MG50388-M
    Tim4 cDNA ORF Clone, Mouse, untagged MG50388-UT
    Tim4 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50388-ACGLN
    Tim4 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50388-ACG
    Tim4 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50388-ACR
    Tim4 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50388-CF
    Tim4 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50388-NF
    Tim4 cDNA ORF Clone, Mouse, C-His tag MG50388-CH
    Tim4 cDNA ORF Clone, Mouse, N-His tag MG50388-NH
    Tim4 cDNA ORF Clone, Mouse, C-HA tag MG50388-CY
    Tim4 cDNA ORF Clone, Mouse, N-HA tag MG50388-NY
    Tim4 cDNA ORF Clone, Mouse, C-Myc tag MG50388-CM
    Tim4 cDNA ORF Clone, Mouse, N-Myc tag MG50388-NM

    Tim4 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    Tim4 cDNA ORF Clone in Cloning Vector, Human HG12161-G
    Tim4 cDNA ORF Clone, Human, untagged HG12161-UT
    Tim4 cDNA ORF Clone, Human, C-GFPSpark® tag HG12161-ACG
    Tim4 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG12161-ACGLN
    Tim4 cDNA ORF Clone, Human, C-OFPSpark® tag HG12161-ACR
    Tim4 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12161-CF
    Tim4 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12161-NF
    Tim4 cDNA ORF Clone, Human, C-His tag HG12161-CH
    Tim4 cDNA ORF Clone, Human, N-His tag HG12161-NH
    Tim4 cDNA ORF Clone, Human, C-HA tag HG12161-CY
    Tim4 cDNA ORF Clone, Human, N-HA tag HG12161-NY
    Tim4 cDNA ORF Clone, Human, C-Myc tag HG12161-CM
    Tim4 cDNA ORF Clone, Human, N-Myc tag HG12161-NM
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