|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human SULT2B1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11410-ACG|
|Human SULT2B1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11410-ACR|
|Human SULT2B1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11410-ANG|
|Human SULT2B1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11410-ANR|
|Human SULT2B1 ORF mammalian expression plasmid, C-Flag tag||HG11410-CF|
|Human SULT2B1 ORF mammalian expression plasmid, C-His tag||HG11410-CH|
|Human SULT2B1 ORF mammalian expression plasmid, C-Myc tag||HG11410-CM|
|Human SULT2B1 ORF mammalian expression plasmid, C-HA tag||HG11410-CY|
|人 SULT2B1 基因全长ORF克隆||HG11410-M|
|Human SULT2B1 ORF mammalian expression plasmid, N-Flag tag||HG11410-NF|
|Human SULT2B1 ORF mammalian expression plasmid, N-His tag||HG11410-NH|
|Human SULT2B1 ORF mammalian expression plasmid, N-Myc tag||HG11410-NM|
|Human SULT2B1 ORF mammalian expression plasmid, N-HA tag||HG11410-NY|
|Human SULT2B1 natural ORF mammalian expression plasmid||HG11410-UT|
|Learn more about expression Vectors|
Sulfotransferase family cytosolic 2B member 1, also known as Sulfotransferase 2B1, ST2B1, Alcohol sulfotransferase, Hydroxysteroid sulfotransferase 2, SULT2B1 and HSST2, is a cytoplasm protein which belongs to the sulfotransferase 1 family. The human hydroxysteroid sulfotransferase (SULT) family is comprised of two subfamilies, SULT2A1 and SULT2B1. SULT2B1 is expressed highly in placenta, prostate and trachea. A lesser expression of SULT1B1 was observed in the small intestine and lung. SULT2B1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. SULT2B1 sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol. The two SULT2B1 isoforms, SULT2B1a and SULT2B1b, are encoded by a single gene as a result of alternative transcription initiation and alternative splicing. SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation.