|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STK16 ORF mammalian expression plasmid, C-GFPSpark tag||HG12201-ACG|
|Human STK16 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12201-ACR|
|Human STK16 ORF mammalian expression plasmid, N-GFPSpark tag||HG12201-ANG|
|Human STK16 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12201-ANR|
|Human STK16 ORF mammalian expression plasmid, C-Flag tag||HG12201-CF|
|Human STK16 ORF mammalian expression plasmid, C-His tag||HG12201-CH|
|Human STK16 ORF mammalian expression plasmid, C-Myc tag||HG12201-CM|
|Human STK16 ORF mammalian expression plasmid, C-HA tag||HG12201-CY|
|Human STK16 Gene cDNA clone plasmid||HG12201-G|
|Human STK16 ORF mammalian expression plasmid, N-Flag tag||HG12201-NF|
|Human STK16 ORF mammalian expression plasmid, N-His tag||HG12201-NH|
|Human STK16 ORF mammalian expression plasmid, N-Myc tag||HG12201-NM|
|Human STK16 ORF mammalian expression plasmid, N-HA tag||HG12201-NY|
|Human STK16 natural ORF mammalian expression plasmid||HG12201-UT|
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Serine/threonine-protein kinase 16, also known as myristoylated and palmitoylated serine/threonine-protein kinase, Protein kinase PKL12, TGF-beta-stimulated factor 1, TSF-1, MPSK1 and STK16, is a membrane protein which is ubiquitously expressed at very low levels. STK16 / MPSK1 belongs to the protein kinase superfamily and Ser/Thr protein kinase family. It contains one protein kinase domain. Transforming growth factor-beta (TGF-beta) shows a variety of biological activities in various organs or cells. Some factors such as Smads (Sma and Mad proteins) and TGF-beta activating kinase 1 have been characterized as signalling molecules downstream of TGF-beta. Several TGF-beta response elements have been identified such as cAMP response element, Smad binding element, and recognition sites for activating protein-1 and stimulating protein-1 in various gene promoters. STK16 / MPSK1 is an unique factor with two biological functions, transcriptional regulation and protein phosphorylation, that may be involved in TGF-beta signals. STK16 / MPSK1 is a protein kinase that act on both serine and threonine residues. STK16 / MPSK1 possessed DNA-binding ability and activated the TGF-beta responsive CNP promoter or vascular endothelial growth factor gene promoter which possesses a sequence element analogous to the TGF-beta responsive GC-rich element of the CNP promoter. STK16 / MPSK1 did not directly activate a Smads-dependent promoter from plasminogen activator inhibitor 1 gene, but it showed enhancement in co-operation with Smad3 and Smad4. STK16 / MPSK1 mRNA as well as its protein level were stimulated by TGF-beta treatment.