Quick Order

Human STAT4 Insect Cell Lysate (WB positive control)

    DatasheetReviewsRelated ProductsProtocols
    Human STAT4 Transfected / Overexpression Cell Lysate Product Information
    Expressed Host:Baculovirus-Insect cells
    Product Description:Baculovirus-Insect Cell lysate that Human STAT4 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
    Sequence information:A DNA sequence encoding the human STAT4 (Q14765) (Met 1-Glu 748) was expressed, with a polyhistidine tag at the N-terminus.
    Predicted N Terminal:Met
    Molecule Mass:The recombinant human STAT4 consists of 767 amino acids and predicts a molecular mass of 88 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
    Species:Human
    Human STAT4 Transfected / Overexpression Cell Lysate Usage Guide
    Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
    Stability:Samples are stable for up to twelve months from date of receipt.
    Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
    Application notes:Western blot (WB): Use at an assay dependent dilution.
    Other Applications: Not tested.
    Optimal dilutions/concentrations should be determined by the end user.
    Human STAT4 References
  • Thierfelder WE, et al. (1996) Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells. Nature. 382(6587):171-4.
  • Hye-Soon L, et al. (2007) Association of STAT4 with Rheumatoid Arthritis in the Korean Population. Mol Med. 13(9-10): 455-60.
  • Chitnis T, et al. (2001) Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis. J Clin Invest. 108(5): 739-47.
  • Size / Price
    Catalog: 13189-H07BL-300
    List Price: 
    Price:      (You Save: )
    Size:
    300 µg
    Quantity:+-
    Availability2 weeks
    Add to CartBulk Discount Inquiry

    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"