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Human ST6GAL1/ST6GAL-I ELISA Pair Set

  • Human ST6GAL1/ST6GAL-I ELISA Pair Set Standard Curve 17182
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Human ST6GAL1 Materials provided
Capture Ab:1 mg/mL of rabbit anti-Human ST6GAL1/ST6GAL-I monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11590-R001)
Detection Ab:0.2 mg/mL of rabbit anti-Human ST6GAL1/ST6GAL-I polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 83 ng of recombinant Human ST6GAL1/ST6GAL-I. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
Human ST6GAL1 Sensitivity
The minimum detectable dose of Human ST6GAL1/ST6GAL-I was determined to be approximately 78.13 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human ST6GAL1 Principle of the product
The Human ST6GAL1/ST6GAL-I ELISA Pair Set is for the quantitative determination of Human ST6GAL1/ST6GAL-I.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human ST6GAL1/ST6GAL-I coated on a 96-well plate. Standards and samples are added to the wells, and any Human ST6GAL1/ST6GAL-I present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human ST6GAL1/ST6GAL-I polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human ST6GAL1/ST6GAL-I present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human ST6GAL1 Storage
ST6GAL1/ST6GAL-I Background

Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

Human ST6GAL1/ST6GAL-I References
  • Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206.
  • Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68.
  • Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414.
  • Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.
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    Datasheet & Documentation

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