|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SLAMF7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11691-ACG|
|Human SLAMF7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11691-ACR|
|Human SLAMF7 ORF mammalian expression plasmid, C-Flag tag||HG11691-CF|
|Human SLAMF7 ORF mammalian expression plasmid, C-His tag||HG11691-CH|
|Human SLAMF7 ORF mammalian expression plasmid, C-Myc tag||HG11691-CM|
|Human SLAMF7 ORF mammalian expression plasmid, C-HA tag||HG11691-CY|
|Human SLAMF7 Gene cDNA clone plasmid||HG11691-G|
|Human SLAMF7 ORF mammalian expression plasmid, Flag tag||HG11691-G-F|
|Human SLAMF7 ORF mammalian expression plasmid, N-Flag tag||HG11691-NF|
|Human SLAMF7 ORF mammalian expression plasmid, N-His tag||HG11691-NH|
|Human SLAMF7 ORF mammalian expression plasmid, N-Myc tag||HG11691-NM|
|Human SLAMF7 ORF mammalian expression plasmid, N-HA tag||HG11691-NY|
|Human SLAMF7 natural ORF mammalian expression plasmid||HG11691-UT|
|Learn more about expression Vectors|
SLAM family member 7 (SLAMF7), also known as CRACC, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. CS1 is a self ligand and homophilic interaction of CS1 regulates NK cell cytolytic activity. CRACC positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, CRACC can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.