SIRT6 cDNA ORF Clone, Human, C-GFPSpark® tag

Cat: HG12156-ACG

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SIRT6 cDNA ORF Clone, Human, C-GFPSpark® tag General Information

Gene

Species
Human
NCBI Ref Seq
RefSeq ORF Size
1068 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human sirtuin 6 with C terminal GFPSpark tag.

Plasmid

Promoter
Enhanced CMV promoter
Restriction Sites
KpnI + XbaI (6kb + 1.8kb)
Tag Sequence
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression

Storage & Shipping

Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

SIRT6 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SIRT6 cDNA ORF Clone, Human, C-GFPSpark® tag Validated Images

Human SIRT6 Gene Expression validated Image 939
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.
other Green fluorescent protein / GFP Gene Plasmid Map 5607

SIRT6 cDNA ORF Clone, Human, C-GFPSpark® tag Alternative Names

SIR2L6 cDNA ORF Clone, Human

SIRT6 Background Information

Sirtuin 6 (SIRT6) is a nicotinamide adenine dinucleotide(+) (NAD(+)) dependent enzyme and stress response protein. SIRT6 has several different functions in multiple different molecular pathways related to DNA repair, glycolysis, gluconeogenesis, tumorigenesis, neurodegeneration, cardiac hypertrophic responses, and more. SIRT6 is a main regulator of metabolism and lifespan and its importance has been implicated in the prevention against aging-related diseases. SIRT6 depletion enhanced chemosensitivity of human hepatoma cells by downregulating MDR1 expression through suppressing C/EBPbeta. SIRT6 may serve as a novel target to enhance chemosensitivity in HCC cells.
Full Name
sirtuin 6
info info
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