PSGL-1 / CD162 qPCR Primer Pairs, Human

Cat: HP100511
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PSGL-1 / CD162 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
PSGL-1 / CD162 qPCR Primer Pairs, Human Alternative Names
CD162 qPCR Primer Pairs, Human;CLA qPCR Primer Pairs, Human;PSGL-1 qPCR Primer Pairs, Human;PSGL1 qPCR Primer Pairs, Human
PSGL-1 / CD162 Background Information

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

Full Name
selectin P ligand
References
  • 1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
  • 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
  • 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
  • 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..
  • 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.
  • PSGL-1 / CD162 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    PSGL-1 / CD162 cDNA ORF Clone in Cloning Vector, Mouse MG50770-G
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, untagged MG50770-UT
    PSGL-1 / CD162 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50770-ACGLN
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50770-ACG
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50770-ACR
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50770-CF
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50770-NF
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-His tag MG50770-CH
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, N-His tag MG50770-NH
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-HA tag MG50770-CY
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, N-HA tag MG50770-NY
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, C-Myc tag MG50770-CM
    PSGL-1 / CD162 cDNA ORF Clone, Mouse, N-Myc tag MG50770-NM

    PSGL-1 / CD162 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    PSGL-1 / CD162 cDNA ORF Clone in Cloning Vector, Human HG10490-M
    PSGL-1 / CD162 cDNA ORF Clone, Human, untagged HG10490-UT
    PSGL-1 / CD162 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10490-ACGLN
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-GFPSpark® tag HG10490-ACG
    PSGL-1 / CD162 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13863-ACGLN
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-OFPSpark® tag HG10490-ACR
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10490-CF
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10490-NF
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-His tag HG10490-CH
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-His tag HG10490-NH
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-HA tag HG10490-CY
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-HA tag HG10490-NY
    PSGL-1 / CD162 cDNA ORF Clone, Human, C-Myc tag HG10490-CM
    PSGL-1 / CD162 cDNA ORF Clone, Human, N-Myc tag HG10490-NM
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