|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SCG2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13441-ACG|
|Human SCG2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13441-ACR|
|Human SCG2 ORF mammalian expression plasmid, C-Flag tag||HG13441-CF|
|Human SCG2 ORF mammalian expression plasmid, C-His tag||HG13441-CH|
|Human SCG2 ORF mammalian expression plasmid, C-Myc tag||HG13441-CM|
|Human SCG2 ORF mammalian expression plasmid, C-HA tag||HG13441-CY|
|Human SCG2 Gene cDNA clone plasmid||HG13441-G|
|Human SCG2 ORF mammalian expression plasmid, N-Flag tag||HG13441-NF|
|Human SCG2 ORF mammalian expression plasmid, N-His tag||HG13441-NH|
|Human SCG2 ORF mammalian expression plasmid, N-Myc tag||HG13441-NM|
|Human SCG2 ORF mammalian expression plasmid, N-HA tag||HG13441-NY|
|Human SCG2 natural ORF mammalian expression plasmid||HG13441-UT|
|Learn more about expression Vectors|
Kit ligand, also known as Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, Kitlg and KITL, is a single-pass type I membrane protein which belongs to the SCF family. KITL / kit ligand also belongs to the family of dimeric transmembrane growth factors. The soluble form of KIT ligand is a secreted protein. Mast cells are thought to participate in a variety of immune responses, such as parasite resistance and the allergic reaction. Mast cell development depends on stem cell factor (Kit ligand) and its receptor, c-Kit. KITL / kit ligand stimulates the proliferation of mast cells. KITL / kit ligand is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Efficient cell surface presentation of KITL / kit ligand is essential for the migration, proliferation, and survival of melanocytes, germ cells, hemopoietic stem cells, and mastocytes. KITL / kit ligand acts synergistically with other cytokines, probably interleukins. KITL / kit ligand plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. It exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. KITL / kit ligand misexpression in some hyperpigmented lesions may open the avenue for Kitl-dependent treatment of pathological skin conditions.