|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG11138-ACG|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG11138-ACR|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-Flag tag||HG11138-CF|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-His tag||HG11138-CH|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-Myc tag||HG11138-CM|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, C-HA tag||HG11138-CY|
|Human S100A8/MRP-8 Gene ORF cDNA clone in cloning vector||HG11138-M|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, N-Flag tag||HG11138-NF|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, N-His tag||HG11138-NH|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, N-Myc tag||HG11138-NM|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid, N-HA tag||HG11138-NY|
|Human S100A8/MRP-8 Gene ORF cDNA clone expression plasmid||HG11138-UT|
|Learn more about expression Vectors|
S100A8 is a member of the S100 protein family containing 2EF-hand calcium-binding motifs. S100 proteins are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of S100A8 protein is associated with various diseases and cancers. S100A8 may have an immunoregulatory role by contributing to the regulation of fetal-maternal interactions. It may play a protective role and its absence may allow infiltration by maternal cells, a process eventually manifesting as resorption. The heterodimeric S100 protein complex S100A8/A9 which has been shown to be involved in inflammatory and neoplastic disorders. The complex can induce cell proliferation, or apoptosis, inflammation, collagen synthesis, and cell migration. S100A8/A9 has emerged as important pro-inflammatory mediator in acute and chronic inflammation. More recently, increased S100A8 and S100A9 levels were also detected in various human cancers, presenting abundant expression in neoplastic tumor cells as well as infiltrating immune cells. On the one hand, S100A8/A9 is a powerful apoptotic agent produced by immune cells, making it a very fascinating tool in the battle against cancer. It spears the risk to induce auto-immune response and may serve as a lead compound for cancer-selective therapeutics. In contrast, S100A8/A9 expression in cancer cells has also been associated with tumor development, cancer invasion or metastasis. Altogether, its expression and potential cytokine-like function in inflammation and in cancer suggests that S100A8/A9 may play a key role in inflammation-associated cancer.