|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human S100A5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11142-ACG|
|Human S100A5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11142-ACR|
|Human S100A5 ORF mammalian expression plasmid, N-GFPSpark tag||HG11142-ANG|
|Human S100A5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11142-ANR|
|Human S100A5 ORF mammalian expression plasmid, C-Flag tag||HG11142-CF|
|Human S100A5 ORF mammalian expression plasmid, C-His tag||HG11142-CH|
|Human S100A5 ORF mammalian expression plasmid, C-Myc tag||HG11142-CM|
|Human S100A5 ORF mammalian expression plasmid, C-HA tag||HG11142-CY|
|Human S100A5 ORF mammalian expression plasmid, N-Flag tag||HG11142-NF|
|Human S100A5 ORF mammalian expression plasmid, N-His tag||HG11142-NH|
|Human S100A5 ORF mammalian expression plasmid, N-Myc tag||HG11142-NM|
|Human S100A5 ORF mammalian expression plasmid, N-HA tag||HG11142-NY|
|Human S100A5 natural ORF mammalian expression plasmid||HG11142-UT|
|Learn more about expression Vectors|
S100 protein?is a family of low molecular weight protein found in vertebrates characterized by two?EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is?100%?soluble in ammonium sulfate?at neutral?pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.?? Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two?EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.