|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human RNASE1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13468-ACG|
|Human RNASE1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13468-ACR|
|Human RNASE1 ORF mammalian expression plasmid, C-Flag tag||HG13468-CF|
|Human RNASE1 ORF mammalian expression plasmid, C-His tag||HG13468-CH|
|Human RNASE1 ORF mammalian expression plasmid, C-Myc tag||HG13468-CM|
|Human RNASE1 ORF mammalian expression plasmid, C-HA tag||HG13468-CY|
|Human RNASE1 Gene cDNA clone plasmid||HG13468-G|
|Human RNASE1 ORF mammalian expression plasmid, N-Flag tag||HG13468-NF|
|Human RNASE1 ORF mammalian expression plasmid, N-His tag||HG13468-NH|
|Human RNASE1 ORF mammalian expression plasmid, N-Myc tag||HG13468-NM|
|Human RNASE1 ORF mammalian expression plasmid, N-HA tag||HG13468-NY|
|Human RNASE1 natural ORF mammalian expression plasmid||HG13468-UT|
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RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.