BIT1 cDNA ORF Clone, Human, C-Myc tag

Cat: HG13645-CM
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BIT1 cDNA ORF Clone, Human, C-Myc tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
540 bp
Description
Full length Clone DNA of Human peptidyl-tRNA hydrolase 2 with C terminal Myc tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-Myc
Tag Sequence
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

BIT1 cDNA ORF Clone, Human, C-Myc tag Alternative Names
BIT1 cDNA ORF Clone, Human;CFAP37 cDNA ORF Clone, Human;CGI-147 cDNA ORF Clone, Human;IMNEPD cDNA ORF Clone, Human;PTH cDNA ORF Clone, Human;PTH 2 cDNA ORF Clone, Human;PTH2 cDNA ORF Clone, Human;PTRH2 cDNA ORF Clone, Human
BIT1 Background Information

PTH2, also known as PTRH2, is a mitochondrial protein which belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.

Full Name
peptidyl-tRNA hydrolase 2
References
  • Prahalad AK. et al., 2006, Proteomics. 6 (12): 3482-93.
  • Jin L. et al., 2000, J Biol Chem. 275 (35): 27238-44.
  • Boone T. et al., 2000, Nat Struct Biol. 7 (6): 486-91.
  • info info
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