PPP2CA cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10420-CF
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PPP2CA cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
930 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human protein phosphatase 2, catalytic subunit, alpha isozyme with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + NotI (6kb + 0.97kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
PPP2CA cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PPP2CA cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human PPP2CA Gene Expression validated Image 1533
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Cynomolgus CDCP1 Gene Plasmid Map 5627
PPP2CA cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
PP2Ac cDNA ORF Clone, Human;PP2CA cDNA ORF Clone, Human;PP2Calpha cDNA ORF Clone, Human;RP-C cDNA ORF Clone, Human
PPP2CA Background Information
Full Name
protein phosphatase 2, catalytic subunit, alpha isozyme

PPP2CA cDNA ORF Clone, Rabbit, Related Products

Product Name Catalog#(PDF)
PPP2CA cDNA ORF Clone in Cloning Vector, Rabbit TG65122-G
PPP2CA cDNA ORF Clone, Rabbit, untagged TG65122-UT
PPP2CA cDNA ORF Clone, Rabbit, C-GFPSpark® tag TG65122-ACG
PPP2CA Lentiviral cDNA ORF Clone, Rabbit, C-GFPSpark® tag TG65122-ACGLN
PPP2CA cDNA ORF Clone, Rabbit, N-GFPSpark® tag TG65122-ANG
PPP2CA cDNA ORF Clone, Rabbit, C-OFPSpark® tag TG65122-ACR
PPP2CA cDNA ORF Clone, Rabbit, N-OFPSpark® tag TG65122-ANR
PPP2CA cDNA ORF Clone, Rabbit, C-DDK (Flag®) tag TG65122-CF
PPP2CA cDNA ORF Clone, Rabbit, N-DDK (Flag®) tag TG65122-NF
PPP2CA cDNA ORF Clone, Rabbit, C-His tag TG65122-CH
PPP2CA cDNA ORF Clone, Rabbit, N-His tag TG65122-NH
PPP2CA cDNA ORF Clone, Rabbit, C-HA tag TG65122-CY
PPP2CA cDNA ORF Clone, Rabbit, N-HA tag TG65122-NY
PPP2CA cDNA ORF Clone, Rabbit, C-Myc tag TG65122-CM
PPP2CA cDNA ORF Clone, Rabbit, N-Myc tag TG65122-NM

PPP2CA cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
PPP2CA cDNA ORF Clone in Cloning Vector, Mouse MG51667-G
PPP2CA cDNA ORF Clone, Mouse, untagged MG51667-UT
PPP2CA cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51667-ACG
PPP2CA Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51667-ACGLN
PPP2CA cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51667-ANG
PPP2CA cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51667-ACR
PPP2CA cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51667-ANR
PPP2CA cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51667-CF
PPP2CA cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51667-NF
PPP2CA cDNA ORF Clone, Mouse, C-His tag MG51667-CH
PPP2CA cDNA ORF Clone, Mouse, N-His tag MG51667-NH
PPP2CA cDNA ORF Clone, Mouse, C-HA tag MG51667-CY
PPP2CA cDNA ORF Clone, Mouse, N-HA tag MG51667-NY
PPP2CA cDNA ORF Clone, Mouse, C-Myc tag MG51667-CM
PPP2CA cDNA ORF Clone, Mouse, N-Myc tag MG51667-NM
PPP2CA qPCR Primer Pairs, Mouse MP201540

PPP2CA cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
PPP2CA cDNA ORF Clone in Cloning Vector, Human HG10420-M
PPP2CA cDNA ORF Clone, Human, untagged HG10420-UT
PPP2CA cDNA ORF Clone, Human, C-GFPSpark® tag HG10420-ACG
PPP2CA Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10420-ACGLN
PPP2CA cDNA ORF Clone, Human, N-GFPSpark® tag HG10420-ANG
PPP2CA cDNA ORF Clone, Human, C-OFPSpark® tag HG10420-ACR
PPP2CA cDNA ORF Clone, Human, N-OFPSpark® tag HG10420-ANR
PPP2CA cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10420-NF
PPP2CA cDNA ORF Clone, Human, C-His tag HG10420-CH
PPP2CA cDNA ORF Clone, Human, N-His tag HG10420-NH
PPP2CA cDNA ORF Clone, Human, C-HA tag HG10420-CY
PPP2CA cDNA ORF Clone, Human, N-HA tag HG10420-NY
PPP2CA cDNA ORF Clone, Human, C-Myc tag HG10420-CM
PPP2CA cDNA ORF Clone, Human, N-Myc tag HG10420-NM
PPP2CA qPCR Primer Pairs, Human HP100450
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