PNLIP qPCR Primer Pairs, Human

Cat: HP102250
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PNLIP qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
PNLIP qPCR Primer Pairs, Human Alternative Names
PL qPCR Primer Pairs, Human;PNLIPD qPCR Primer Pairs, Human;PTL qPCR Primer Pairs, Human
PNLIP Background Information

PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Full Name
pancreatic lipase
References
  • Hegele RA, et al. (2001) Polymorphisms in PNLIP, encoding pancreatic lipase, and associations with metabolic traits. J Hum Genet. 46(6):320-4.
  • Thomas A, et al. (2005) Role of the lid hydrophobicity pattern in pancreatic lipase activity. J Biol Chem. 280(48):40074-83.
  • Colin DY, et al. (2008) Exploring the active site cavity of human pancreatic lipase. Biochem Biophys Res Commun. 370(3):394-8.
  • PNLIP qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    PNLIP cDNA ORF Clone in Cloning Vector, Rhesus CG90537-U
    PNLIP cDNA ORF Clone, Rhesus, untagged CG90537-UT
    PNLIP Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90537-ACGLN
    PNLIP cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90537-ACG
    PNLIP cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90537-ACR
    PNLIP cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90537-CF
    PNLIP cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90537-NF
    PNLIP cDNA ORF Clone, Rhesus, C-His tag CG90537-CH
    PNLIP cDNA ORF Clone, Rhesus, N-His tag CG90537-NH
    PNLIP cDNA ORF Clone, Rhesus, C-HA tag CG90537-CY
    PNLIP cDNA ORF Clone, Rhesus, N-HA tag CG90537-NY
    PNLIP cDNA ORF Clone, Rhesus, C-Myc tag CG90537-CM
    PNLIP cDNA ORF Clone, Rhesus, N-Myc tag CG90537-NM

    PNLIP qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    PNLIP cDNA ORF Clone in Cloning Vector, Rat RG83426-U
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    PNLIP qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    PNLIP cDNA ORF Clone in Cloning Vector, Mouse MG53859-U
    PNLIP cDNA ORF Clone, Mouse, untagged MG53859-UT
    PNLIP cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53859-ACG
    PNLIP Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53859-ACGLN
    PNLIP cDNA ORF Clone, Mouse, C-OFPSpark® tag MG53859-ACR
    PNLIP cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53859-CF
    PNLIP cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG53859-NF
    PNLIP cDNA ORF Clone, Mouse, C-His tag MG53859-CH
    PNLIP cDNA ORF Clone, Mouse, N-His tag MG53859-NH
    PNLIP cDNA ORF Clone, Mouse, C-HA tag MG53859-CY
    PNLIP cDNA ORF Clone, Mouse, N-HA tag MG53859-NY
    PNLIP cDNA ORF Clone, Mouse, C-Myc tag MG53859-CM
    PNLIP cDNA ORF Clone, Mouse, N-Myc tag MG53859-NM

    PNLIP qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    PNLIP cDNA ORF Clone in Cloning Vector, Human HG13564-G
    PNLIP cDNA ORF Clone, Human, untagged HG13564-UT
    PNLIP cDNA ORF Clone, Human, C-GFPSpark® tag HG13564-ACG
    PNLIP Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13564-ACGLN
    PNLIP cDNA ORF Clone, Human, C-OFPSpark® tag HG13564-ACR
    PNLIP cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13564-CF
    PNLIP cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13564-NF
    PNLIP cDNA ORF Clone, Human, C-His tag HG13564-CH
    PNLIP cDNA ORF Clone, Human, N-His tag HG13564-NH
    PNLIP cDNA ORF Clone, Human, C-HA tag HG13564-CY
    PNLIP cDNA ORF Clone, Human, N-HA tag HG13564-NY
    PNLIP cDNA ORF Clone, Human, C-Myc tag HG13564-CM
    PNLIP cDNA ORF Clone, Human, N-Myc tag HG13564-NM
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