|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PMM2 ORF mammalian expression plasmid, C-GFPSpark tag||HG14648-ACG|
|Human PMM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14648-ACR|
|Human PMM2 ORF mammalian expression plasmid, N-GFPSpark tag||HG14648-ANG|
|Human PMM2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14648-ANR|
|Human PMM2 ORF mammalian expression plasmid, C-Flag tag||HG14648-CF|
|Human PMM2 ORF mammalian expression plasmid, C-His tag||HG14648-CH|
|Human PMM2 ORF mammalian expression plasmid, C-Myc tag||HG14648-CM|
|Human PMM2 ORF mammalian expression plasmid, C-HA tag||HG14648-CY|
|Human PMM2 Gene cDNA clone plasmid||HG14648-G|
|Human PMM2 ORF mammalian expression plasmid, N-Flag tag||HG14648-NF|
|Human PMM2 ORF mammalian expression plasmid, N-His tag||HG14648-NH|
|Human PMM2 ORF mammalian expression plasmid, N-Myc tag||HG14648-NM|
|Human PMM2 ORF mammalian expression plasmid, N-HA tag||HG14648-NY|
|Human PMM2 natural ORF mammalian expression plasmid||HG14648-UT|
|Learn more about expression Vectors|
Phosphomannomutase 2, also known as PMM2 and CDG1, belongs to the eukaryotic PMM family. Phosphomannomutase 2 catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate. Mannose 1-phosphate is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. GDP-mannose can transfer its small sugar molecule called mannose to the growing oligosaccharide chain. Once the correct number of small sugar molecules are linked together to form the oligosaccharide, it can be attached to a protein. Phosphomannomutase 2 is also required for a number of critical mannosyl transfer reactions. Mutations in PMM2 gene have been shown to cause defects in the protein glycosylation pathway manifest as carbohydrate-deficient glycoprotein syndrome type I.