PKM2 qPCR Primer Pairs, Human

Cat: HP101307
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PKM2 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
PKM2 qPCR Primer Pairs, Human Alternative Names
CTHBP qPCR Primer Pairs, Human;HEL-S-30 qPCR Primer Pairs, Human;OIP3 qPCR Primer Pairs, Human;PK3 qPCR Primer Pairs, Human;PKM2 qPCR Primer Pairs, Human;TCB qPCR Primer Pairs, Human;THBP1 qPCR Primer Pairs, Human
PKM2 Background Information

Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Full Name
pyruvate kinase, muscle
References
  • Bluemlein K, et al. (2011) No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2 (5): 393-400.
  • Gupta V, et al. (2010) Dominant Negative Mutations Affect Oligomerization of Human Pyruvate Kinase M2 Isozyme and Promote Cellular Growth and Polyploidy. J Biol Chem. 285 (22): 16864-73.
  • Eigenbrodt E, et al. (1992) Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells. Crit Rev Oncog. 3 (1): 91-115.
  • PKM2 qPCR Primer Pairs, Rabbit, Related Products

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    PKM2 cDNA ORF Clone in Cloning Vector, Rabbit TG65061-G
    PKM2 cDNA ORF Clone, Rabbit, untagged TG65061-UT
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    PKM2 Lentiviral cDNA ORF Clone, Rabbit, C-GFPSpark® tag TG65061-ACGLN
    PKM2 cDNA ORF Clone, Rabbit, N-GFPSpark® tag TG65061-ANG
    PKM2 cDNA ORF Clone, Rabbit, C-OFPSpark® tag TG65061-ACR
    PKM2 cDNA ORF Clone, Rabbit, N-OFPSpark® tag TG65061-ANR
    PKM2 cDNA ORF Clone, Rabbit, C-DDK (Flag®) tag TG65061-CF
    PKM2 cDNA ORF Clone, Rabbit, N-DDK (Flag®) tag TG65061-NF
    PKM2 cDNA ORF Clone, Rabbit, C-His tag TG65061-CH
    PKM2 cDNA ORF Clone, Rabbit, N-His tag TG65061-NH
    PKM2 cDNA ORF Clone, Rabbit, C-HA tag TG65061-CY
    PKM2 cDNA ORF Clone, Rabbit, N-HA tag TG65061-NY
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    PKM2 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    PKM2 cDNA ORF Clone in Cloning Vector, Rat RG80568-U
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    PKM2 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80568-ACG
    PKM2 cDNA ORF Clone, Rat, N-GFPSpark® tag RG80568-ANG
    PKM2 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80568-ACR
    PKM2 cDNA ORF Clone, Rat, N-OFPSpark® tag RG80568-ANR
    PKM2 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80568-CF
    PKM2 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80568-NF
    PKM2 cDNA ORF Clone, Rat, C-His tag RG80568-CH
    PKM2 cDNA ORF Clone, Rat, N-His tag RG80568-NH
    PKM2 cDNA ORF Clone, Rat, C-HA tag RG80568-CY
    PKM2 cDNA ORF Clone, Rat, N-HA tag RG80568-NY
    PKM2 cDNA ORF Clone, Rat, C-Myc tag RG80568-CM
    PKM2 cDNA ORF Clone, Rat, N-Myc tag RG80568-NM

    PKM2 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    PKM2 cDNA ORF Clone in Cloning Vector, Mouse MG53948-U
    PKM2 cDNA ORF Clone in Cloning Vector, Mouse MG51121-G
    PKM2 cDNA ORF Clone, Mouse, untagged MG51121-UT
    PKM2 cDNA ORF Clone, Mouse, untagged MG53948-UT
    PKM2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51121-ACGLN
    PKM2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53948-ACGLN
    PKM2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53948-ACG
    PKM2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51121-ACG
    PKM2 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51121-ANG
    PKM2 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG53948-ANG
    PKM2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51121-ACR
    PKM2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG53948-ACR
    PKM2 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51121-ANR
    PKM2 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG53948-ANR
    PKM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51121-CF
    PKM2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53948-CF
    PKM2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG53948-NF
    PKM2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51121-NF
    PKM2 cDNA ORF Clone, Mouse, C-His tag MG51121-CH
    PKM2 cDNA ORF Clone, Mouse, C-His tag MG53948-CH
    PKM2 cDNA ORF Clone, Mouse, N-His tag MG51121-NH
    PKM2 cDNA ORF Clone, Mouse, N-His tag MG53948-NH
    PKM2 cDNA ORF Clone, Mouse, C-HA tag MG53948-CY
    PKM2 cDNA ORF Clone, Mouse, C-HA tag MG51121-CY
    PKM2 cDNA ORF Clone, Mouse, N-HA tag MG51121-NY
    PKM2 cDNA ORF Clone, Mouse, N-HA tag MG53948-NY
    PKM2 cDNA ORF Clone, Mouse, C-Myc tag MG51121-CM
    PKM2 cDNA ORF Clone, Mouse, C-Myc tag MG53948-CM
    PKM2 cDNA ORF Clone, Mouse, N-Myc tag MG53948-NM
    PKM2 cDNA ORF Clone, Mouse, N-Myc tag MG51121-NM

    PKM2 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    PKM2 cDNA ORF Clone in Cloning Vector, Human HG11430-M
    PKM2 cDNA ORF Clone in Cloning Vector, Human HG16269-G
    PKM2 cDNA ORF Clone, Human, untagged HG11430-UT
    PKM2 cDNA ORF Clone, Human, untagged HG16269-UT
    PKM2 cDNA ORF Clone, Human, C-GFPSpark® tag HG11430-ACG
    PKM2 cDNA ORF Clone, Human, C-GFPSpark® tag HG16269-ACG
    PKM2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG16269-ACGLN
    PKM2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11430-ACGLN
    PKM2 cDNA ORF Clone, Human, N-GFPSpark® tag HG11430-ANG
    PKM2 cDNA ORF Clone, Human, N-GFPSpark® tag HG16269-ANG
    PKM2 cDNA ORF Clone, Human, C-OFPSpark® tag HG16269-ACR
    PKM2 cDNA ORF Clone, Human, C-OFPSpark® tag HG11430-ACR
    PKM2 cDNA ORF Clone, Human, N-OFPSpark® tag HG11430-ANR
    PKM2 cDNA ORF Clone, Human, N-OFPSpark® tag HG16269-ANR
    PKM2 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG16269-CF
    PKM2 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11430-CF
    PKM2 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11430-NF
    PKM2 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG16269-NF
    PKM2 cDNA ORF Clone, Human, C-His tag HG16269-CH
    PKM2 cDNA ORF Clone, Human, C-His tag HG11430-CH
    PKM2 cDNA ORF Clone, Human, N-His tag HG16269-NH
    PKM2 cDNA ORF Clone, Human, N-His tag HG11430-NH
    PKM2 cDNA ORF Clone, Human, C-HA tag HG16269-CY
    PKM2 cDNA ORF Clone, Human, C-HA tag HG11430-CY
    PKM2 cDNA ORF Clone, Human, N-HA tag HG11430-NY
    PKM2 cDNA ORF Clone, Human, N-HA tag HG16269-NY
    PKM2 cDNA ORF Clone, Human, C-Myc tag HG16269-CM
    PKM2 cDNA ORF Clone, Human, C-Myc tag HG11430-CM
    PKM2 cDNA ORF Clone, Human, N-Myc tag HG16269-NM
    PKM2 cDNA ORF Clone, Human, N-Myc tag HG11430-NM
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