PDPK1 cDNA ORF Clone, Human, N-DDK (Flag®) tag

Cat: HG10681-NF
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PDPK1 cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
1671 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human 3-phosphoinositide dependent protein kinase-1 with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-FLAG
Restriction Sites
KpnI + XbaI (6kb + 1.71kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
PDPK1 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PDPK1 cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
Human aFGF/FGF1 Gene Plasmid Map 5637
Human PDPK1 Gene Expression validated Image 16447
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PDPK1 cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
PDK1 cDNA ORF Clone, Human;PDPK2 cDNA ORF Clone, Human;PDPK2P cDNA ORF Clone, Human;PRO0461 cDNA ORF Clone, Human
PDPK1 Background Information
Full Name
3-phosphoinositide dependent protein kinase 1

PDPK1 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
PDPK1 cDNA ORF Clone in Cloning Vector, Mouse MG50856-G
PDPK1 cDNA ORF Clone, Mouse, untagged MG50856-UT
PDPK1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50856-ACG
PDPK1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50856-ACGLN
PDPK1 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50856-ANG
PDPK1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50856-ACR
PDPK1 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50856-ANR
PDPK1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50856-CF
PDPK1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50856-NF
PDPK1 cDNA ORF Clone, Mouse, C-His tag MG50856-CH
PDPK1 cDNA ORF Clone, Mouse, N-His tag MG50856-NH
PDPK1 cDNA ORF Clone, Mouse, C-HA tag MG50856-CY
PDPK1 cDNA ORF Clone, Mouse, N-HA tag MG50856-NY
PDPK1 cDNA ORF Clone, Mouse, C-Myc tag MG50856-CM
PDPK1 cDNA ORF Clone, Mouse, N-Myc tag MG50856-NM
PDPK1 qPCR Primer Pairs, Mouse MP200827

PDPK1 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
PDPK1 cDNA ORF Clone in Cloning Vector, Human HG10681-M
PDPK1 cDNA ORF Clone, Human, untagged HG10681-UT
PDPK1 cDNA ORF Clone, Human, C-GFPSpark® tag HG10681-ACG
PDPK1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10681-ACGLN
PDPK1 cDNA ORF Clone, Human, N-GFPSpark® tag HG10681-ANG
PDPK1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10681-ACR
PDPK1 cDNA ORF Clone, Human, N-OFPSpark® tag HG10681-ANR
PDPK1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10681-CF
PDPK1 cDNA ORF Clone, Human, C-His tag HG10681-CH
PDPK1 cDNA ORF Clone, Human, N-His tag HG10681-NH
PDPK1 cDNA ORF Clone, Human, C-HA tag HG10681-CY
PDPK1 cDNA ORF Clone, Human, N-HA tag HG10681-NY
PDPK1 cDNA ORF Clone, Human, C-Myc tag HG10681-CM
PDPK1 cDNA ORF Clone, Human, N-Myc tag HG10681-NM
PDPK1 qPCR Primer Pairs, Human HP100644
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