Human PBK Baculovirus-Insect cells Overexpression Lysate

Cat: 10729-H08BL
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All PBK Reagents
CRO Services
Product recommended

All PBK Reagents
Human PBK Baculovirus-Insect cells Overexpression Lysate Product Information
Product Description
This Human PBK overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of PBK protein (Cat: 10729-H08B) from the overexpression lysate was verified.
Expression Host
Baculovirus-Insect cells
Species
Human
Sequence Information
A DNA sequence encoding the full length of human PBK (NP_060962.2) (Met 1-Val 322) was fused with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human PBK consists of 333 amino acids and predicts a molecular mass of 37 kDa as estimated in SDS-PAGE under reducing conditions.
Human PBK Baculovirus-Insect cells Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Human PBK Baculovirus-Insect cells Overexpression Lysate Alternative Names
Human CT84 Overexpression Lysate;Human HEL164 Overexpression Lysate;Human Nori-3 Overexpression Lysate;Human SPK Overexpression Lysate;Human TOPK Overexpression Lysate
PBK Background Information

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.

Full Name
PDZ binding kinase
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
  • info info
    添加购物车成功! 添加购物车失败!