|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PDIA4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13333-ACG|
|Human PDIA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13333-ACR|
|Human PDIA4 ORF mammalian expression plasmid, C-Flag tag||HG13333-CF|
|Human PDIA4 ORF mammalian expression plasmid, C-His tag||HG13333-CH|
|Human PDIA4 ORF mammalian expression plasmid, C-Myc tag||HG13333-CM|
|Human PDIA4 ORF mammalian expression plasmid, C-HA tag||HG13333-CY|
|Human PDIA4 Gene cDNA clone plasmid||HG13333-G|
|Human PDIA4 ORF mammalian expression plasmid, N-Flag tag||HG13333-NF|
|Human PDIA4 ORF mammalian expression plasmid, N-His tag||HG13333-NH|
|Human PDIA4 ORF mammalian expression plasmid, N-Myc tag||HG13333-NM|
|Human PDIA4 ORF mammalian expression plasmid, N-HA tag||HG13333-NY|
|Human PDIA4 natural ORF mammalian expression plasmid||HG13333-UT|
|Learn more about expression Vectors|
ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein which belongs to the protein disulfide isomerase family. ERP72 is a stress protein and participates in the catalysis of protein-S-S-bond rearrangement. Both of PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.