|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human PCSK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10129-ACG|
|Human PCSK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10129-ACR|
|Human PCSK1 ORF mammalian expression plasmid, C-Flag tag||HG10129-CF|
|Human PCSK1 ORF mammalian expression plasmid, C-His tag||HG10129-CH|
|Human PCSK1 ORF mammalian expression plasmid, C-Myc tag||HG10129-CM|
|Human PCSK1 ORF mammalian expression plasmid, C-HA tag||HG10129-CY|
|Human PCSK1 Gene cDNA clone plasmid||HG10129-M|
|Human PCSK1 natural ORF mammalian expression plasmid||HG10129-M-N|
|Human PCSK1 ORF mammalian expression plasmid, N-Flag tag||HG10129-NF|
|Human PCSK1 ORF mammalian expression plasmid, N-His tag||HG10129-NH|
|Human PCSK1 ORF mammalian expression plasmid, N-Myc tag||HG10129-NM|
|Human PCSK1 ORF mammalian expression plasmid, N-HA tag||HG10129-NY|
|Human PCSK1 natural ORF mammalian expression plasmid||HG10129-UT|
|Learn more about expression Vectors|
Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.