|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human NLK ORF mammalian expression plasmid, C-GFPSpark tag||HG11573-ACG|
|Human NLK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11573-ACR|
|Human NLK ORF mammalian expression plasmid, N-GFPSpark tag||HG11573-ANG|
|Human NLK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11573-ANR|
|Human NLK ORF mammalian expression plasmid, C-Flag tag||HG11573-CF|
|Human NLK ORF mammalian expression plasmid, C-His tag||HG11573-CH|
|Human NLK ORF mammalian expression plasmid, C-Myc tag||HG11573-CM|
|Human NLK ORF mammalian expression plasmid, C-HA tag||HG11573-CY|
|Human NLK Gene cDNA clone plasmid||HG11573-G|
|Human NLK ORF mammalian expression plasmid, N-Flag tag||HG11573-NF|
|Human NLK ORF mammalian expression plasmid, N-His tag||HG11573-NH|
|Human NLK ORF mammalian expression plasmid, N-Myc tag||HG11573-NM|
|Human NLK ORF mammalian expression plasmid, N-HA tag||HG11573-NY|
|Human NLK natural ORF mammalian expression plasmid||HG11573-UT|
|Learn more about expression Vectors|
Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.