|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NLGN3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11160-ACG|
|Human NLGN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11160-ACR|
|Human NLGN3 ORF mammalian expression plasmid, C-Flag tag||HG11160-CF|
|Human NLGN3 ORF mammalian expression plasmid, C-His tag||HG11160-CH|
|Human NLGN3 ORF mammalian expression plasmid, C-Myc tag||HG11160-CM|
|Human NLGN3 ORF mammalian expression plasmid, C-HA tag||HG11160-CY|
|Human NLGN3 ORF mammalian expression plasmid, Flag tag||HG11160-M-F|
|Human NLGN3 ORF mammalian expression plasmid, N-Flag tag||HG11160-NF|
|Human NLGN3 ORF mammalian expression plasmid, N-His tag||HG11160-NH|
|Human NLGN3 ORF mammalian expression plasmid, N-Myc tag||HG11160-NM|
|Human NLGN3 ORF mammalian expression plasmid, N-HA tag||HG11160-NY|
|Human NLGN3 natural ORF mammalian expression plasmid||HG11160-UT|
|Learn more about expression Vectors|
Neuroligin 3 (NLGN3) is a member of the type-B carboxylesterase/lipase family. Neuroligins (NLGNs) are a family of presumptive postsynaptic cell adhesion molecules. Neuroligins (NLs) constitute a family of cell-surface proteins that interact with neurexins (beta-Nxs), another class of neuronal cell-surface proteins, one of each class functioning together in synapse formation. Neuroligins control the formation and functional balance of excitatory and inhibitory synapses in hippocampal neurons. NLGN1 and NLGN2 isoforms are concentrated at glutamatergic and GABAergic synapses, respectively, but the cellular expression and synaptic localization of the endogenous. NLGN3 was enriched in brain, where NLGN3 protein levels increased during postnatal development, coinciding with the peak of synaptogenesis. The NLGN3 is a synaptic adhesion molecule that is a shared component of glutamatergic and GABAergic synapses. Mutations in NLGN3 gene may be associated with autism and Asperger syndrome.