NGF qPCR Primer Pairs, Human

Cat: HP101278
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NGF qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
NCBI RefSeq:
PCR Size:
130bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
NGF qPCR Primer Pairs, Human Alternative Names
Beta-NGF qPCR Primer Pairs, Human;HSAN5 qPCR Primer Pairs, Human;NGFB qPCR Primer Pairs, Human
NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Full Name
nerve growth factor (beta polypeptide)
References
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
  • NGF qPCR Primer Pairs, Canine, Related Products

    Product Name Catalog#(PDF)
    NGF cDNA ORF Clone in Cloning Vector, Canine DG70027-G
    NGF cDNA ORF Clone, Canine, untagged DG70027-UT
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    NGF cDNA ORF Clone, Canine, C-DDK (Flag®) tag DG70027-CF
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    NGF cDNA ORF Clone, Canine, C-His tag DG70027-CH
    NGF cDNA ORF Clone, Canine, N-His tag DG70027-NH
    NGF cDNA ORF Clone, Canine, C-HA tag DG70027-CY
    NGF cDNA ORF Clone, Canine, N-HA tag DG70027-NY
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    NGF qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    NGF cDNA ORF Clone in Cloning Vector, Rat RG80426-G
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    NGF cDNA ORF Clone, Rat, C-OFPSpark® tag RG80426-ACR
    NGF cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80426-CF
    NGF cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80426-NF
    NGF cDNA ORF Clone, Rat, C-His tag RG80426-CH
    NGF cDNA ORF Clone, Rat, N-His tag RG80426-NH
    NGF cDNA ORF Clone, Rat, C-HA tag RG80426-CY
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    NGF qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    NGF cDNA ORF Clone in Cloning Vector, Mouse MG50385-M
    NGF cDNA ORF Clone, Mouse, untagged MG50385-UT
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    NGF cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50385-ACG
    NGF cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50385-ACR
    NGF cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50385-CF
    NGF cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50385-NF
    NGF cDNA ORF Clone, Mouse, C-His tag MG50385-CH
    NGF cDNA ORF Clone, Mouse, N-His tag MG50385-NH
    NGF cDNA ORF Clone, Mouse, C-HA tag MG50385-CY
    NGF cDNA ORF Clone, Mouse, N-HA tag MG50385-NY
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    NGF qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    NGF cDNA ORF Clone, Human, untagged HG11050-C
    NGF cDNA ORF Clone, Human, untagged HG11050-UT
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    NGF Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11050-ACGLN
    NGF cDNA ORF Clone, Human, C-OFPSpark® tag HG11050-ACR
    NGF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11050-CF
    NGF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11050-NF
    NGF cDNA ORF Clone, Human, C-His tag HG11050-CH
    NGF cDNA ORF Clone, Human, N-His tag HG11050-NH
    NGF cDNA ORF Clone, Human, C-HA tag HG11050-CY
    NGF cDNA ORF Clone, Human, N-HA tag HG11050-NY
    NGF cDNA ORF Clone, Human, C-Myc tag HG11050-CM
    NGF cDNA ORF Clone, Human, N-Myc tag HG11050-NM
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