MTOR cDNA ORF Clone, Human, C-OFPSpark® tag

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MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
7650 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human mechanistic target of rapamycin (serine/threonine kinase) with C terminal OFPSpark / RFP tag.
Plasmid
Promoter
Enhanced CMV promoter
Restriction Sites
HindIII + NotI (6kb + 8.34kb)
Tag Sequence
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

MTOR cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: Validated Images

MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: Alternative Names

FRAP cDNA ORF Clone, Human; FRAP1 cDNA ORF Clone, Human; FRAP2 cDNA ORF Clone, Human; RAFT1 cDNA ORF Clone, Human; RAPT1 cDNA ORF Clone, Human; SKS cDNA ORF Clone, Human

MTOR Background Information

The protein encoded by MTOR gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Full Name
mechanistic target of rapamycin (serine/threonine kinase)
References
  • Zhao J, Zhai B, Gygi SP, Goldberg AL. mTOR inhibition activates overall protein degradation by the ubiquitin proteasome system as well as by autophagy. Proceedings of the National Academy of Sciences of the United States of America. 2015;112(52):15790-15797.
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