C1orf33 cDNA ORF Clone, Human, C-His tag General Information
Gene
Description
Full length Clone DNA of Human mRNA turnover 4 homolog (S. cerevisiae) with C terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
C1orf33 cDNA ORF Clone, Human, C-His tag Alternative Names
C1orf33 cDNA ORF Clone, Human;dJ657E11.4 cDNA ORF Clone, Human;MRT4 cDNA ORF Clone, Human
C1orf33 Background Information
MRTO4, also known as MRT4, belongs to the ribosomal protein L10P family. MRTO4 is a ribosomal P0-like protein showing extensive sequence similarity to the ribosomal P0 protein. The precise function of MRTO4 is currently unknown. It appears to be involved in mRNA turnover and ribosome assembly. MRTO4 acts as a trans-acting factor which modulates the assembly of the pre-60S particle.
Full Name
MRT4 homolog, ribosome maturation factor
References
Zhao J. et al., 2011, J Proteomics. 75 (2): 588-602. Havugimana PC. et al., 2012, Cell. 150 (5): 1068-81. Wu Z. et al., 2012, PLoS One. 7 (8): e43997. Michalec B. et al., 2010, Int J Biochem Cell Biol. 42 (5): 736-48.
