METTL1 qPCR Primer Pairs, Human

Cat: HP101395
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METTL1 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
METTL1 qPCR Primer Pairs, Human Alternative Names
C12orf1 qPCR Primer Pairs, Human;TRM8 qPCR Primer Pairs, Human;TRMT8 qPCR Primer Pairs, Human;YDL201w qPCR Primer Pairs, Human
METTL1 Background Information

tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Full Name
methyltransferase like 1
References
  • Bahr, A.et al., 1999, Genomics. 57 (3):424-8.
  • Wikman, H. et al., 2005,Genes Chromosomes Cancer. 42 (2):193-9.
  • Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.
  • METTL1 qPCR Primer Pairs, Rat, Related Products

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    METTL1 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    METTL1 cDNA ORF Clone in Cloning Vector, Human HG11525-M
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    METTL1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11525-ACGLN
    METTL1 cDNA ORF Clone, Human, N-GFPSpark® tag HG11525-ANG
    METTL1 cDNA ORF Clone, Human, C-OFPSpark® tag HG11525-ACR
    METTL1 cDNA ORF Clone, Human, N-OFPSpark® tag HG11525-ANR
    METTL1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11525-CF
    METTL1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11525-NF
    METTL1 cDNA ORF Clone, Human, C-His tag HG11525-CH
    METTL1 cDNA ORF Clone, Human, N-His tag HG11525-NH
    METTL1 cDNA ORF Clone, Human, C-HA tag HG11525-CY
    METTL1 cDNA ORF Clone, Human, N-HA tag HG11525-NY
    METTL1 cDNA ORF Clone, Human, C-Myc tag HG11525-CM
    METTL1 cDNA ORF Clone, Human, N-Myc tag HG11525-NM
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