|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG11372-ACG|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG11372-ACR|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-Flag tag||HG11372-CF|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-His tag||HG11372-CH|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-Myc tag||HG11372-CM|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, C-HA tag||HG11372-CY|
|Human MDGA2 Gene ORF cDNA clone in cloning vector||HG11372-M|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, N-Flag tag||HG11372-NF|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, N-His tag||HG11372-NH|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, N-Myc tag||HG11372-NM|
|Human MDGA2 Gene ORF cDNA clone expression plasmid, N-HA tag||HG11372-NY|
|Human MDGA2 Gene ORF cDNA clone expression plasmid||HG11372-UT|
|Learn more about expression Vectors|
Mouse MAM domain-containing glycosylphosphatidylinositol anchor protein 2, also known as MAM domain-containing protein 1, MDGA2 and MAMDC1, is a cell membrane protein which contains six Ig-like (immunoglobulin-like) domains and one MAM domain. Analyses of the full-length coding region of MDGA1 and MDGA2 indicate that they encode proteins that comprise a novel subgroup of the Ig superfamily and have a unique structural organization consisting of six immunoglobulin (Ig)-like domains followed by a single MAM domain. Biochemical characterization demonstrates that MDGA1 and MDGA2 proteins are highly glycosylated, and that MDGA1 is tethered to the cell membrane by a GPI anchor. The MDGAs are differentially expressed by subpopulations of neurons in both the central and peripheral nervous systems, including neurons of the basilar pons, inferior olive, cerebellum, cerebral cortex, olfactory bulb, spinal cord, and dorsal root and trigeminal ganglia. The similarity of MDGAs to other Ig-containing molecules and their temporal-spatial patterns of expression within restricted neuronal populations, for example migrating pontine neurons and D1 spinal interneurons, suggest a role for these novel proteins in regulating neuronal migration, as well as other aspects of neural development, including axon guidance.