CD146 / MCAM qPCR Primer Pairs, Human

Cat: HP100026
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CD146 / MCAM Reagents
CRO Services
Product recommended

All CD146 / MCAM Reagents
CD146 / MCAM qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
NCBI RefSeq:
PCR Size:
150bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CD146 / MCAM qPCR Primer Pairs, Human Validated Images
Human CD146/MCAM qPCR 2685
MCAM dissolution curves
Human CD146/MCAM qPCR 2686
MCAM amplification curves
CD146 / MCAM qPCR Primer Pairs, Human Alternative Names
CD146 qPCR Primer Pairs, Human;MUC18 qPCR Primer Pairs, Human
CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
  • CD146 / MCAM qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD146 / MCAM cDNA ORF Clone in Cloning Vector, Mouse MG50794-G
    CD146 / MCAM cDNA ORF Clone, Mouse, untagged MG50794-UT
    CD146 / MCAM Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50794-ACGLN
    CD146 / MCAM cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50794-ACG
    CD146 / MCAM cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50794-ACR
    CD146 / MCAM cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50794-CF
    CD146 / MCAM cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50794-NF
    CD146 / MCAM cDNA ORF Clone, Mouse, C-His tag MG50794-CH
    CD146 / MCAM cDNA ORF Clone, Mouse, N-His tag MG50794-NH
    CD146 / MCAM cDNA ORF Clone, Mouse, C-HA tag MG50794-CY
    CD146 / MCAM cDNA ORF Clone, Mouse, N-HA tag MG50794-NY
    CD146 / MCAM cDNA ORF Clone, Mouse, C-Myc tag MG50794-CM
    CD146 / MCAM cDNA ORF Clone, Mouse, N-Myc tag MG50794-NM

    CD146 / MCAM qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    CD146 / MCAM cDNA ORF Clone in Cloning Vector, Human HG10115-M
    CD146 / MCAM cDNA ORF Clone, Human, untagged HG10115-UT
    CD146 / MCAM Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10115-ACGLN
    CD146 / MCAM cDNA ORF Clone, Human, C-GFPSpark® tag HG10115-ACG
    CD146 / MCAM cDNA ORF Clone, Human, C-OFPSpark® tag HG10115-ACR
    CD146 / MCAM cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10115-CF
    CD146 / MCAM cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10115-NF
    CD146 / MCAM cDNA ORF Clone, Human, C-His tag HG10115-CH
    CD146 / MCAM cDNA ORF Clone, Human, N-His tag HG10115-NH
    CD146 / MCAM cDNA ORF Clone, Human, C-HA tag HG10115-CY
    CD146 / MCAM cDNA ORF Clone, Human, N-HA tag HG10115-NY
    CD146 / MCAM cDNA ORF Clone, Human, C-Myc tag HG10115-CM
    CD146 / MCAM cDNA ORF Clone, Human, N-Myc tag HG10115-NM
    info info
    添加购物车成功! 添加购物车失败!