|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LYG1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13457-ACG|
|Human LYG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13457-ACR|
|Human LYG1 ORF mammalian expression plasmid, C-Flag tag||HG13457-CF|
|Human LYG1 ORF mammalian expression plasmid, C-His tag||HG13457-CH|
|Human LYG1 ORF mammalian expression plasmid, C-Myc tag||HG13457-CM|
|Human LYG1 ORF mammalian expression plasmid, C-HA tag||HG13457-CY|
|Human LYG1 Gene cDNA clone plasmid||HG13457-G|
|Human LYG1 ORF mammalian expression plasmid, N-Flag tag||HG13457-NF|
|Human LYG1 ORF mammalian expression plasmid, N-His tag||HG13457-NH|
|Human LYG1 ORF mammalian expression plasmid, N-Myc tag||HG13457-NM|
|Human LYG1 ORF mammalian expression plasmid, N-HA tag||HG13457-NY|
|Human LYG1 natural ORF mammalian expression plasmid||HG13457-UT|
|Learn more about expression Vectors|
Lysozyme G-like 1 belongs to the glycosyl hydrolase 23 family. Glycoside hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. Lysozyme G-like 1 exhibits hydrolase activity, acting on glycosyl bonds (inferred); lysozyme activity (inferred). It is found in extracellular region and may functions in cell wall macromolecule catabolic process, metabolic process and peptidoglycan catabolic process. The lysozyme G gene structure has been largely conserved during vertebrate evolution, except at the 5' end of the gene, which varies in number of exons.