Lactoferrin cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG11096-CF
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All Lactoferrin Reagents
CRO Services
Product recommended

All Lactoferrin Reagents
Lactoferrin cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
2172 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1824 C/T not causing the amino acid variation.
Description
Full length Clone DNA of Human lactotransferrin with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI(6kb+2.17kb )
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Lactoferrin cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Lactoferrin cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5609
Human Lactotransferrin/LTF Gene Expression validated Image 14249
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
Lactoferrin cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
GIG12 cDNA ORF Clone, Human;HEL110 cDNA ORF Clone, Human;HLF2 cDNA ORF Clone, Human;LF cDNA ORF Clone, Human
Lactoferrin Background Information

Lactotransferrin, also known as Lactoferrin, Talalactoferrin and LTF, is a secreted protein which belongs to the transferrin family. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and lactoferroxin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.

Full Name
lactotransferrin
References
  • Sánchez L, et al.,1992, Arch. Dis. Child. 67 (5): 657 - 61.
  • Wakabayashi H, et al., 2000, J. Antimicrob. Chemother. 46 (4): 595-602.
  • Nozaki A, et al., 2003, J. Biol. Chem. 278 (12): 10162-73.
  • Azzam HS, et al., 2007, Liver Int. 27 (1): 17-25.
  • Lactoferrin cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    Lactoferrin cDNA ORF Clone in Cloning Vector, Mouse MG50750-G
    Lactoferrin cDNA ORF Clone, Mouse, untagged MG50750-UT
    Lactoferrin Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50750-ACGLN
    Lactoferrin cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50750-ACG
    Lactoferrin cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50750-ACR
    Lactoferrin cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50750-CF
    Lactoferrin cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50750-NF
    Lactoferrin cDNA ORF Clone, Mouse, C-His tag MG50750-CH
    Lactoferrin cDNA ORF Clone, Mouse, N-His tag MG50750-NH
    Lactoferrin cDNA ORF Clone, Mouse, C-HA tag MG50750-CY
    Lactoferrin cDNA ORF Clone, Mouse, N-HA tag MG50750-NY
    Lactoferrin cDNA ORF Clone, Mouse, C-Myc tag MG50750-CM
    Lactoferrin cDNA ORF Clone, Mouse, N-Myc tag MG50750-NM
    Lactoferrin qPCR Primer Pairs, Mouse MP200725

    Lactoferrin cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Lactoferrin cDNA ORF Clone in Cloning Vector, Human HG11096-M
    Lactoferrin cDNA ORF Clone, Human, untagged HG11096-UT
    Lactoferrin cDNA ORF Clone, Human, C-GFPSpark® tag HG11096-ACG
    Lactoferrin Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11096-ACGLN
    Lactoferrin cDNA ORF Clone, Human, C-OFPSpark® tag HG11096-ACR
    Lactoferrin cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11096-NF
    Lactoferrin cDNA ORF Clone, Human, C-His tag HG11096-CH
    Lactoferrin cDNA ORF Clone, Human, N-His tag HG11096-NH
    Lactoferrin cDNA ORF Clone, Human, C-HA tag HG11096-CY
    Lactoferrin cDNA ORF Clone, Human, N-HA tag HG11096-NY
    Lactoferrin cDNA ORF Clone, Human, C-Myc tag HG11096-CM
    Lactoferrin cDNA ORF Clone, Human, N-Myc tag HG11096-NM
    Lactoferrin qPCR Primer Pairs, Human HP101011
    info info
    添加购物车成功! 添加购物车失败!