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Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tag

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Human KNG1 cDNA Clone Product Information
NCBI RefSeq:NM_001102416.2
RefSeq ORF Size:1935bp
cDNA Description:Full length Clone DNA of Homo sapiens kininogen 1, transcript variant 1 with N terminal HA tag.
Gene Synonym:BDK, KNG
Species:Human
Vector:pCMV3-SP-N-HA
Plasmid:
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tag on other vectors
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10529-ACG$245
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10529-ACR$245
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10529-CF$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tagHG10529-CH$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10529-CM$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA tagHG10529-CY$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10529-M$75
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10529-M-F$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10529-M-N$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10529-NF$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His tagHG10529-NH$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10529-NM$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tagHG10529-NY$215
Human KNG1/Kininogen 1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10529-UT$215
 Learn more about expression Vectors
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Background

Mouse kininogen-1, also known as high molecular weight kininogen, williams-Fitzgerald-Flaujeac factor, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, KNG1 and BDK, is a secreted protein which contains three cystatin domains. Kininogen-1 / KNG1 is a protein from the blood coagulation system as well as the kinin-kallikrein system. It is a protein that adsorbs to the surface of biomaterials that come in contact with blood. Kininogen-1 / KNG1 circulates throughout the blood and quickly adsorbs to the material surfaces. Kininogen-1 / KNG1 is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. Kininogen-1 / KNG1 is one of the kininogens, a class of proteins. As with many other coagulation proteins, the protein was initially named after the patients in whom deficiency was first observed. When the clinical data were combined, it turned out that all patients, in fact, had a deficiency of the same protein. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) which is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

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