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Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set

  • Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set
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Human F11R Materials provided
Capture Ab:0.5 mg/mL of mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10198-MM08)
Detection Ab:0.1 mg/mL of mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.4 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10198-MM02)
Standard:Each vial contains 10 ng of recombinant Human Junctional Adhesion Molecule A / JAM-A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
Human F11R Sensitivity
The minimum detectable dose of Human Junctional Adhesion Molecule A / JAM-A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human F11R Principle of the product
The Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set is for the quantitative determination of Human Junctional Adhesion Molecule A / JAM-A.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Junctional Adhesion Molecule A / JAM-A coated on a 96-well plate. Standards and samples are added to the wells, and any Human Junctional Adhesion Molecule A / JAM-A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Junctional Adhesion Molecule A / JAM-A monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Junctional Adhesion Molecule A / JAM-A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human F11R Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human F11R ELISA Pair Set Standard Curve
Junctional Adhesion Molecule A Background

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

Human Junctional Adhesion Molecule A References
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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