Human Immunodeficiency Virus type 1 gp140 Protein (His Tag)

Human Immunodeficiency Virus Type 1gp140 Protein Datasheet

HIV-1 gp140 Protein Product Information

• Synonym: Gp140
• Protein Construction: A DNA sequence encoding the HIV-1 envelope glycoprotein gp160 extracellular domain (Val30-Tyr677)，termed as gp140, was fused with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
• Source: Human Immunodeficiency Virus type 1 (HIV-1)
• Strain: Similar to 97CN001
• Expression Host: Human Cells

HIV-1 gp140 Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to biotinylated human CD4-his (Cat:10400-H08H) in functional Elisa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 30
• Molecular Mass: The recombinant HIV-1 gp140 consists of 694 amino acids after removal of the signal peptide and has a predicted molecular mass of 78.2 kDa. The apparent molecular mass of the recombinant protein is approximately 119 KDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: HIV-1 gp140 protein

HIV-1 gp140 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HIV-1 gp140 Protein Description

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses.The HIV-1 envelope glycoprotein gp160, also known as Glycoprotein 160, is cleaved into two chains: the surface protein gp120 and the transmembrane protein gp41. The mature envelope protein (Env) consists of a homotrimer of non-covalently associated gp120-gp41 heterodimers. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Surface protein gp120 is a ligand for CD209 / DC-SIGN and CLEC4M / DC-SIGNR. It may target the virus to gut-associated lymphoid tissue (GALT) by binding host ITGA4/ITGB7 (alpha-4/beta-7 integrins), a complex that mediates T-cell migration to the GALT. The transmembrane protein gp41 (TM) acts as a class I viral fusion protein, and membranes fusion leads to delivery of the nucleocapsid into the cytoplasm. The external domains of the HIV-1 envelope glycoprotein (gp120 and the gp41 ectodomain, collectively known as gp140) contain all known viral neutralization epitopes.

References

1. Robertson, DL. et al.,1995, J. Mol. Evol. 40 (3): 249-59.
2. Gallo SA., et al., 2003, Biochim. Biophys. Acta. 1614: 36-50.
3. Bobkov, AF. et al., 2004,?J. Med. Virol. 74 (2): 191-6.
4. Yang X., J. et al., 2005, Virol. 79: 12132-47.
5. Beddows, S. et al., 2007, Virology. 360 (2): 329-40.
6. Du, SX. et al., 2009, Virology. 395 (1): 33-44.
7. Mendu, DR. et al., 2007, Biochem Biophys Res Commun. 363 (3): 466-71.