Human Immunodeficiency Virus type 1 gp120 / SU Protein (His Tag)

Human Immunodeficiency Virus type 1 / HIV-1 gp120 Protein

HIV-1 Glycoprotein 120 / gp120 / SU Protein Product Information

• Synonym: gp120, SU, Surface glycoprotein 120
• Protein Construction: A DNA sequence encoding the HIV-1 surface glycoprotein gp120 ( Ala 29 - Glu 503 ), which is cleaved from the full length envelope glycoprotein gp160, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus
• Source: Human Immunodeficiency Virus type 1 (HIV-1)
• Strain: Similar to 97CN001
• Expression Host: Human Cells

HIV-1 Glycoprotein 120 / gp120 / SU Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 29
• Molecular Mass: The recombinant HIV-1 gp120 consists of 486 amino acids after removal of the signal peptide and has a predicted molecular mass of 54.6 kDa. The apparent molecular mass of the recombinant protein is approximately 80-100 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: gp120 protein

HIV-1 Glycoprotein 120 / gp120 / SU Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HIV-1 Glycoprotein 120 / gp120 / SU Protein Description

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses.
The HIV-1 surface protein gp120, also known as Glycoprotein 120, SU, and gp120 is not anchored to the viral envelope, but associates with the extravirion surface through its binding to TM. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Surface protein gp120 is a ligand for CD209 / DC-SIGN and CLEC4M / DC-SIGNR. It may target the virus to gut-associated lymphoid tissue (GALT) by binding host ITGA4/ITGB7 (alpha-4/beta-7 integrins), a complex that mediates T-cell migration to the GALT. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.

References

1. Robertson, DL.et al., 1995, J. Mol. Evol. 40 (3): 249-59.
2. Gallo SA., et al., 2003, Biochim. Biophys. Acta. 1614: 36-50. 
3. Bobkov, AF. et al., 2004, J. Med. Virol. 74 (2): 191-6.
4. Yang X., J. et al., 2005, Virol. 79: 12132-47.
5. Hemelaar, J. et al., 2006, AIDS. 20 (16): W13-23. 
6. Zhou T., et al., 2007, Nature. 445:732-7.   
7. Miyauchi K., et al., 2009, Cell. 137:433-44.