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Human ITGAV ORF mammalian expression plasmid, C-His tag

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Human ITGAV cDNA Clone Product Information
NCBI RefSeq:NM_002210.4
RefSeq ORF Size:3147bp
cDNA Description:Full length Clone DNA of Homo sapiens integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) with C terminal His tag.
Gene Synonym:CD51, MSK8, VNRA, DKFZp686A08142, ITGAV
Species:Human
Vector:pCMV3-C-His
Plasmid:pCMV3-ITGAV-His
Restriction Site:KpnI + XbaI (6kb + 3.19kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 87 C/T, 2028 C/T not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human ITGAV Gene Plasmid Map
Human ITGAV natural ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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Catalog: HG11269-CH
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