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Human IL4 / Interleukin-4 Protein

  • Human IL4/IL-4/Interleukin-4 Protein 14106
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 IL4 Protein Product Information
Synonym:BCGF-1,BCGF1,BSF-1,BSF1,IL-4,Interleukin-4
Protein Construction:A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His25-Ser153) was expressed and purified, with an initial Met.
Species:Human
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 IL4 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of Recombinant Human IL-4 is approximately 3.2x10E4 IU/μg.
Endotoxin:< 10 EU per mg protein as determined by the LAL method.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.
Formulation:Lyophilized from sterile 50 mM Tris, 300 mM NaCl, pH 7.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 IL4 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL4 Related Pathways

    Other IL4 Recombinant Protein Products
    IL4/IL-4/Interleukin-4 Background

    Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

     IL4/IL-4/Interleukin-4 References
  • Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
  • Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
  • Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
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    Catalog: GMP-11846-HNAE-20
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    Datasheet & Documentation

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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"